AutoESDCas: A Web-Based Tool for the Whole-Workflow Editing Sequence Design for Microbial Genome Editing Based on the CRISPR/Cas System

Genome editing is the basis for the modification of engineered
microbes. In the process of genome editing, the design of editing
sequences, such as primers and sgRNA, is very important for the accurate
positioning of editing sites and efficient sequence editing. The whole
process of genome editing involves multiple rounds and types of editing
sequence design, while the development of related whole-workflow design
tools for high-throughput experimental requirements lags. Here, we
propose AutoESDCas, an online tool for the end-to-end editing sequence
design for microbial genome editing based on the CRISPR/Cas system.
This tool facilitates all types of genetic manipulation covering diverse
experimental requirements and design scenarios, enables biologists
to quickly and efficiently obtain all editing sequences needed for
the entire genome editing process, and empowers high-throughput strain
modification. Notably, with its off-target risk assessment function
for editing sequences, the usability of the design results is significantly
improved. AutoESDCas is freely available at https://autoesdcas.biodesign.ac.cn/with the source code at https://github.com/tibbdc/AutoESDCas/.