Overcoming clinical resistance to EZH2 inhibition using rational epigenetic combination therapy

Supplementary data for Kazansky et al, "Overcoming clinical resistance to EZH2 inhibition using rational epigenetic combination therapy" and "Epigenetic targeting of PGBD5-dependent DNA damage in SMARCB1-deficient sarcomas." Raw RNA-seq data from G401 cells can be found at the Gene Expression Omnibus (GEO) repository, accession number GSE213845. RNA-seq data from patient tumor samples has been deposited to the Database of Genotypes and Phenotypes (dbGaP), accession number phs003188.v1.p1. All files are labeled with their corresponding figures. "DESeq_processing_FINAL.R" was used for analysis of patient RNA-seq data, using "20230131_SampleTable.csv" and all "*_htseq.txt" files as input. "Tumor_growth_analysis_FINAL_UPDATED" was used for analysis of tumor growth kinetics using the aucVardiTest function. This was used for both of the manuscripts desscribed above.本数据集为Kazansky等人发表的《通过合理表观遗传联合疗法克服EZH2抑制剂临床耐药性》以及《SMARCB1缺失肉瘤中PGBD5依赖性DNA损伤的表观遗传靶向治疗》两篇论文的补充数据。G401细胞的原始RNA测序（RNA-seq）数据可于基因表达综合数据库（Gene Expression Omnibus, GEO）中获取，登录号为GSE213845。患者肿瘤样本的RNA测序数据已提交至基因型与表型数据库（Database of Genotypes and Phenotypes, dbGaP），登录号为phs003188.v1.p1。 所有文件均标注有对应的图表编号。 患者RNA测序数据的分析采用"DESeq_processing_FINAL.R"脚本，以"20230131_SampleTable.csv"及所有"*_htseq.txt"文件作为输入文件。 "Tumor_growth_analysis_FINAL_UPDATED"脚本借助aucVardiTest函数用于肿瘤生长动力学分析，上述两篇论文均采用了该分析流程。