Association and disassociation constants for SHP-1 and SHP-2 binding to G6B-b phosphopeptides.

Kon and Koff (s-1) were determined using Prism 5 software (GraphPad Software, California, U.S.A) at a variety of concentrations from Biacore 3000 runs carried out on three different days. Kd values = M.借助美国加利福尼亚州GraphPad软件公司开发的Prism 5软件，基于三天内完成的多轮Biacore 3000实验所获取的系列浓度数据，计算得到结合速率常数（Kon）与解离速率常数（Koff，单位：s⁻¹）。解离常数（Kd）的单位为摩尔（M）。