Determination of transcript's polyA tail length in Wild-Type and Mov10 KO mouse ESCs.

The RNA helicase MOV10 targets mRNAs to promote their decay. MOV10 can directly bind to N6-methyladenosine (m6A) nucleotides, the most prevalent type of mRNA internal modification in mammals. Here we investigate the mechanism by which MOV10 promotes decay by looking at changes in the polyA tail of its targets. PolyA tail length shortening is usually associated with transcript decay. However, we did not observe changes in the polyA profile for MOV10 targets in MOV10-depleted and Wild-Type controls. The observation was true for targeted transcripts containing the m6A modification and for targeted transcripts without the modification. We conclude that MOV10-mediated decay does not perturb the polyA tail dynamics of its targets in ESCs. Direct RNAseq between Wild-type and KO mouse ESCs