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Sample numbers, barcode information, primer information and project name from bacteria samples collected in the Caribbean during 2013 (Contagious coral diseases project)

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DataONE2025-03-09 更新2025-04-26 收录
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<p>This dataset contains sample information, barcode information, primer information and the project name.</p> <p>Coral disease transmission experiments were completed for dark-spot syndrome on Sidereastrea siderea&nbsp;and yellow-band disease on Orbicella faveolata, as described in Randall et al. 2016.&nbsp;Following experimentation, microbial communities were extracted from tissue samples to determine whether any&nbsp;potential pathogen may have transmitted from healthy to exposed corals. Microbial communities on healthy corals&nbsp;were compared with diseased corals to identify any potential pathogens.</p> <p>Experimental diseased and healthy corals were sampled and their microbial communities were analyzed using 454 Illumina pyrosequencing&nbsp;of the amplified 16S rRNA gene on the V1-V3&nbsp;hypervariable region.</p>

本数据集包含样本信息、条形码(barcode)信息、引物(primer)信息以及项目名称。 本数据集针对星孔珊瑚(Sidereastrea siderea)的黑斑综合征与团块珊瑚(Orbicella faveolata)的黄带病完成了珊瑚疾病传播实验,实验细节如Randall等人2016年的研究所述。实验结束后,研究人员从珊瑚组织样本中提取微生物群落,以确定是否存在潜在病原菌从健康珊瑚传播至暴露珊瑚体内。通过对比健康珊瑚与患病珊瑚的微生物群落,以甄别潜在病原菌。 研究人员对实验用患病及健康珊瑚进行采样,并针对扩增得到的16S rRNA基因V1-V3高变区,采用454 Illumina焦磷酸测序技术对其微生物群落展开分析。
创建时间:
2025-03-09
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