A high-throughput assay for quantitative measurement of PCR errors

The project employs unique molecular identifier tagging technique and high-throughput sequencing to study PCR amplification errors. In total, 10 different PCR assays involving 9 different polymerases were studied. Two experiments were conduced to describe errors introduced during linear PCR and 20-25 cycles of conventional PCR, both ran in two replicates (independent library preparations and sequencing). All experiments were performed for the same 150-bp long template, amplification-free control with the same template is also provided. Each sequencing read includes an UMI tag and unique sample barcode used for filtering cross-sample contamination, for details on data processing and analysis see https://github.com/mikessh/polyfid.