3DED/MicroED datasets of hemozoin recorded by a SINGLA detector

32 diffraction datasets were collected using a Thermo Scientific Glacios operated at 200 kV with a stage temperature of ~80 K. The gun lens was reduced to the minimum (8), and spot size (11) with a C2 aperture of 50 µm was used. The beam was brought to parallel corresponding to a beam diameter of approximately 1.7 µm. The diffraction images were recorded by a DECTRIS SINGLA detector in the h5 format. NeXus format header files were subsequently generated using Nexgen (https://github.com/dials/nexgen). The diffraction patterns were collected at a nominal camera length of 677 mm according to the microscope control software (estimated to be to 809 mm at the detector). The sample was rotated through 90° over 18s with the camera collecting data at 50 Hz, producing images representing 0.1°. Subsequently, the oscillation angle was refined to 0.11°. The dose rate under these conditions was 0.01 e- Å-2 s-1. A data processing script is provided to index, integrate and scale 64 lattices across the datasets using DIALS 3.13本研究共采集32套衍射数据集，实验使用赛默飞世尔科技（Thermo Scientific）Glacios透射电子显微镜，设备工作电压设置为200 kV，样品台温度维持在约80 K。将电子枪透镜调至最小档位（档位8），采用光斑尺寸档位11，并搭配孔径为50 µm的C2光阑。调整电子束至平行状态，对应束斑直径约为1.7 µm。衍射图像由DECTRIS SINGLA探测器以h5格式记录。后续通过Nexgen（https://github.com/dials/nexgen）生成NeXus格式头文件。根据电镜控制软件标注，数据采集时的名义相机长度为677 mm（探测器位置处估算值为809 mm）。样品在18秒内完成90°旋转，探测器以50 Hz的频率采集数据，单张图像对应0.1°的转角。随后将振荡角修正为0.11°。该实验条件下的电子剂量率为0.01 e⁻·Å⁻²·s⁻¹。本数据集附带数据处理脚本，可通过DIALS 3.13对所有数据集内的64个晶格完成索引、积分与缩放处理。