High-throughput mapping of long-range neuronal projection using in situ sequencing, Chen et al., dataset 3

Raw in situ sequencing data and other images for the paper of the same name. Part of four related datasets:
10.17632/mk82s9x82t.1
10.17632/g7kdxznt6w.1
10.17632/86wf7xfz5x.1
10.17632/2w649fccnt.1

These datasets demonstrate BARseq, a highly multiplexed projection mapping method based on in situ sequencing of RNA barcodes. These data further demonstrates the use of BARseq to correlate gene expression and projections at cellular resolution with high throughput, and the use of BARseq to map projections of Cre-labeled subpopulation of neurons.
These datasets Also contain two single-cell RNAseq datasets. One single-cell RNAseq dataset identified four Intratelencephalic neuron subtypes in mouse auditory cortex. A second single-cell RNAseq dataset compared endogenous gene expression in barcoded vs. non-barcoded neurons and found that information about endogenous gene expression can be successfully recovered from the barcoded neurons.

Description of files in the current dataset:

Dataset 3:
XC75-345 images and processing script.zip: Images and processing script for registering and processing XC75BARseq data. Both original max projection images and registered/processed images are provided. script.m processes the images and produces the xc75 data matrix in "BARseq and FISH data analysis.zip".

本数据集为同名论文配套的原始原位测序（in situ sequencing）数据及其他图像，属于4组关联数据集之一，关联数据集的DOI编号分别为10.17632/mk82s9x82t.1、10.17632/g7kdxznt6w.1、10.17632/86wf7xfz5x.1及10.17632/2w649fccnt.1。
这些数据集展示了BARseq技术——一种基于RNA条形码原位测序的高度多重投影标绘方法。相关数据进一步验证了BARseq技术可在单细胞分辨率下以高通量方式关联基因表达与神经元投射，同时可用于标绘Cre标记的神经元亚群投射。
本数据集还包含两组单细胞RNA测序（single-cell RNAseq）数据集：其一为在小鼠听觉皮层中鉴定出4种脑内神经元亚型的单细胞RNA测序数据集；其二为对比条形码标记与未标记神经元内源性基因表达的数据集，实验结果证实可从条形码标记神经元中成功恢复其内源基因表达信息。
当前数据集的文件说明如下：
数据集3：
XC75-345 images and processing script.zip：用于配准与处理XC75系列BARseq数据的图像及处理脚本。本压缩包同时提供原始最大投影图像与经配准/处理后的图像，脚本script.m可对图像进行处理，并在"BARseq与荧光原位杂交（FISH）数据分析.zip"中生成xc75数据矩阵。