An Organelle-Directed Chemical Ligation Approach Enables Dual-Color Detection of Mitophagy
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https://tandf.figshare.com/articles/dataset/An_Organelle-Directed_Chemical_Ligation_Approach_Enables_Dual-Color_Detection_of_Mitophagy/13567002/1
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Dysfunctional organelles and defective turnover of organelles are engaged in multiple human diseases, but are elusive to image with conventional organelle probes. To overcome this, we developed intra-mitochondrial CLICK to assess mitophagy (IMCLAM), using a pair of conventional ΔΨm probes, where each probe alone fails to track dysfunctional mitochondria. The <i>in situ</i> formed optical triad is stably trapped in mitochondria without resorting to ΔΨm. Utilizing an acidity-responsive ΔΨm probe, IMCLAM enabled fluorescence-on detection of mitophagy by sensing pH acidification upon delivery of mitochondria into lysosomes. Moreover, we applied IMCLAM to assay mitophagy induced by pharmacological compounds in living cells and wild-type zebrafish embryos. Thus, IMCLAM offers a simplified tool to study mitochondria and mitophagy and provide a basis for screening mitophagy-inducing compounds.
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提供机构：
Taylor & Francis
创建时间：
2021-01-13
