GENE ASSEMBLY IN AGROBACTERIUM VIA NUCLEIC ACID TRANSFER USING RECOMBINASE TECHNOLOGY (GAANTRY)

This article examines gene assembly in Agrobacterium via nucleic acid transfer using recombinase technology (GAANTRY). It explores the molecular mechanisms underlying recombinase-mediated gene stacking and its application in plant genetic engineering. Special attention is given to the efficiency, precision, and scalability of GAANTRY systems in comparison to traditional transformation methods. The study highlights the role of site-specific recombinases in assembling multiple gene constructs into the T-DNA region of Agrobacterium tumefaciens. Furthermore, the article discusses the advantages of this technology in developing transgenic plants with complex traits, such as stress resistance and improved metabolic pathways. The findings demonstrate that GAANTRY represents a powerful and flexible tool for modern biotechnology and agricultural innovation.本文针对利用重组酶技术通过核酸转移实现农杆菌（Agrobacterium）基因组装的GAANTRY技术展开研究，探讨了重组酶介导的基因堆叠的分子机制，以及该技术在植物基因工程中的应用。相较于传统转化方法，本研究重点考察了GAANTRY系统的转化效率、精准性与可扩展性。本研究着重阐明了位点特异性重组酶在将多基因构建体组装至根癌农杆菌（Agrobacterium tumefaciens）的T-DNA（转移DNA）区域中的作用。此外，本文还探讨了该技术在培育具有复杂性状（如抗逆性与改良代谢途径）的转基因植物方面的优势。研究结果表明，GAANTRY是一款适用于现代生物技术与农业创新领域的强大且灵活的工具。