Genome-wide expression changes in response to the treatment of anti-CD93 in mouse tumor endothelial cells

We isolated ECs from anti-CD93 antibody treated or control KPC tumor and performed RNA-seq to investigate genome-wide expression changes in response to the treatment of anti-CD93. Many of the genes affected by anti-CD93 were functionally related to cell adhesion, angiogenesis or vasculature development. Gene ontology analysis further confirmed that the transcriptomic changes by anti-CD93 were mainly related to biological pathways of angiogenesis, cell adhesion, or vasculature development. Overall design: Total RNA was extracted and purified using QIAGEN RNeasy Mini Kit (Cat.74104) from tumor endothelial cells, which were isolated by FACS sorting (CD31+CD45- population) from established subcutaneous KPC tumor after 48 hours of antibody treatment. The mRNA libraries were constructed using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Cat. 634411). 150bp paired-end RNA sequencing was performed on Illumina NovaSEQ 6000 platform and the RNA-seq data were generated by the Genomics and Microarray Shared Resource at UCCC.