HLY-02-01 Primary Production (ASCII) [Hill, V.]

Primary productivity was measured by 14C (carbon) uptake fixation with simulated in situ (SIS) incubations. Isotope stocks were prepared according to the recommendations of Fitzwater et al., 1982. Our SIS incubator had neutral density and/or blue plastic filters to simulate in situ irradiance spectra at depths consistent with sample collection. Temperature was regulated with flowing surface seawater. Samples were placed in 280ml polycarbonate bottles and inoculated with 370 kBq 14C-NaHCO3 and incubated for short period (S) 6-8 hours and long period (L) ~24 hours. Total activity added and particulate adsorption was measured at time zero in parallel samples. Particulate material was harvested on 25mm Whatman GF/F filters and rinsed with 5-10ml of 0.01 N HCL in filtered seawater to remove inorganic carbon. Radioactivity was assayed by liquid scintillation counting and corrected for particulate adsorption at time zero, background and counting efficiency. Dataset includes hourly (mg C m-3 h-1) and daily rates (mg C m-2 d-1) of 14C uptake measured with both short and long incubation periods with matching chlorophyll (mg m- 3) measuring using the fluorometric method of Holm-Hansen (1965) by Dean Stockwell of the University of Alaska, Fairbanks. These ASCII data were collected aboard the U.S. Coast Guard Cutter (USCGC) Healy cruise HLY-02-01.

本数据集采用14C（碳）摄取固定法结合模拟原位（Simulated In Situ, SIS）培养法测定海洋初级生产力。同位素储备液的配制遵循Fitzwater等人1982年的研究建议。本研究使用的SIS培养装置配备中性密度滤膜和/或蓝色塑料滤膜，用以模拟与样品采集深度匹配的原位辐照度光谱。培养温度通过流动表层海水进行调控。样品置于280毫升聚碳酸酯瓶中，接种370千贝可（kBq）的14C-碳酸氢钠（14C-NaHCO3），分别设置短期（S，6~8小时）与长期（L，约24小时）两种培养时长。在平行样品的零时刻，测定添加的总放射性活度与颗粒吸附量。颗粒物质通过25毫米直径的Whatman GF/F滤膜收集，随后用5~10毫升经过滤海水中配制的0.01当量盐酸（0.01 N HCl）淋洗，以去除无机碳。放射性活度采用液体闪烁计数法测定，并针对零时刻颗粒吸附、本底辐射与计数效率进行校正。数据集包含两种培养时长下测得的14C摄取速率：小时尺度速率单位为mg C m⁻³ h⁻¹（毫克碳每立方米每小时），日尺度速率单位为mg C m⁻² d⁻¹（毫克碳每平方米每天）；同时配套有叶绿素浓度数据（单位为mg m⁻³，毫克每立方米），该数据由阿拉斯加大学费尔班克斯分校的Dean Stockwell采用Holm-Hansen（1965）荧光法测定。本ASCII格式数据集采集自美国海岸警卫队希利号破冰船（USCGC Healy）HLY-02-01航次。