A post-Pleistocene calibrated mutation rate from insect museum specimens

Quantifying the age of recent species divergence events can be challenging in the absence of calibration points within many groups. The katydid species <i>Neoconocephalus lyristes</i> provides the opportunity to calibrate a post-Pleistocene mutation rate using a known biogeographic event. DNA was extracted from pinned specimens of <i>N. lyristes</i> from both Midwest and Atlantic populations and the mitochondrial gene COI sequenced using primers designed from extant specimens. Coalescent analyses using both strict and relaxed molecular clock models were performed in BEAST v1.8.2. The assumption of a strict molecular clock could not be rejected in favor of the relaxed clock model as the distribution of the standard deviation of the clock rate strongly abutted zero. The strict molecular clock model resulted in an intraspecific calculated mutation rate of 1.65 %/myr, a value within the range observed in other studies of insect mitochondrial mutation rates.