Oxysterols[M1] Suppress Release of DNA from Granulocytes into Extracellular Space After Stimulation with PhorbolMyristate Acetate[M1]The article title is different from the one submitted online at susy.mdpi.com. Please confirm which one is correct.

In the present study, we examined the effects of four oxysterols on the release of DNA and proteins from neutrophils. Unexpectedly, oxysterols, but not cholesterol, suppressed DNA release from neutrophils. Furthermore, treatment of cells with methyl-β-cyclodextrin (MβCD) that extracts sterol compounds from cell membranes to form inclusion complexes enhanced DNA release from PMA-stimulated cells. These observations suggest that oxysterols may act as modulators of neutrophil activation.本研究考察了四种氧固醇（oxysterols）对中性粒细胞释放脱氧核糖核酸（DNA）与蛋白质的影响。出乎意料的是，氧固醇而非胆固醇可抑制中性粒细胞的DNA释放。此外，使用可从细胞膜提取甾醇类化合物并形成包合复合物的甲基-β-环糊精（methyl-β-cyclodextrin, MβCD）处理细胞，可增强佛波酯（PMA）刺激的细胞的DNA释放量。上述观测结果表明，氧固醇或可作为中性粒细胞活化的调节因子。