Raw data behavior in olfactory tubing maze and open field

Preclinical and clinical studies show that mild to moderate hypothermia is neuroprotective in sudden cardiac arrest, ischemic stroke, perinatal hypoxia/ischemia, traumatic brain injury and seizures. Induction of hypothermia largely involves physical cooling therapies, which induce several clinical complications, while some molecules have shown to be efficient in pharmacologically induced hypothermia (PIH). Neurotensin (NT), a 13 amino-acid neuropeptide that regulates body temperature, interacts with various receptors to mediate its peripheral and central effects. NT induces PIH when administered intracerebrally. However, these effects are not observed if NT is administered peripherally, due to its rapid degradation and poor passage of the blood brain barrier (BBB). We conjugated NT to peptides that bind the low-density lipoprotein receptor (LDLR) to generate “vectorized” forms of NT with enhanced BBB permeability. We evaluated their effects in epileptic conditions following peripheral a..., Olfactory Tubing Maze (OTM)
The hippocampus-dependent learning and memory performance of control mice injected with saline solution (SHAM), SE and SE + VH-N412 mice were assessed 3 weeks after SE (12-week-old male mice). Behavioral assessment, based on the OTM, was carried out as we previously described (Roman et al., 2002; Girard et al., 2014) and we have shown that the FVB/N mouse strain used in the present study performed very well in this task (Girard et al., 2016). Briefly, mice were trained to learn odor-reward associations. The mice placed on water restriction were submitted to the presentation of odors in testing chambers (TC). Two synthetic odors (strawberry and jasmine), coming from 2 distinct arms and randomly assigned to these arms, were simultaneously presented to the mice. Each of these 2 odors was arbitrarily associated either with a drop of water (the reward) or with a non-aversive but unpleasant sound, which were delivered depending on the arm chosen by the mice. The OT..., , # Raw data behavior in olfactory tubing maze and open field

[https://doi.org/10.5061/dryad.6wwpzgn8k](https://doi.org/10.5061/dryad.6wwpzgn8k)

## Description of the data and file structure

Epilepsy is associated with learning and memory difficulties in patients and animal models. We thus assessed whether our hypothermia inducing molecule VH-N412-induced reduction of neurodegeneration and inflammation ameliorates mnesic capacities of our epileptic animals. Three weeks after status epilepticus, a group of mice was submitted to behavioral tests. We evaluated hippocampus-dependent learning and memory performance using the olfactory tubing maze (OTM) and the inter-trial interval (ITI). Exploratory and spontaneous locomotor activity of the same mice was assessed in the open-field paradigm where the average distances covered by mice from the different groups were evaluated.

### Files and variables

#### File: Behavior\_RAW\_DATA\_OTM\_and\_OPEN\_FIELD.xlsx

**Description:** Evaluate the m...

临床前与临床研究均证实，轻中度低温对心搏骤停、缺血性脑卒中、围产期缺氧缺血、创伤性脑损伤及癫痫发作具有神经保护作用。低温诱导多依赖物理降温疗法，但该疗法可引发多种临床并发症；而部分分子已被证实可有效实现药物诱导低温（pharmacologically induced hypothermia, PIH）。
神经降压素（Neurotensin, NT）是一种调控体温的13肽神经肽，可通过结合多种受体介导其外周与中枢生物学效应。脑内给予神经降压素可诱导药物诱导低温，但由于其快速降解且难以透过血脑屏障（blood brain barrier, BBB），外周给药时无法观察到此类效应。本研究将神经降压素与可结合低密度脂蛋白受体（low-density lipoprotein receptor, LDLR）的肽段偶联，制备得到血脑屏障穿透性增强的“载体化”神经降压素变体。本研究通过外周给药方式评估了该变体在癫痫模型中的效应。嗅觉管道迷宫（Olfactory Tubing Maze, OTM）
本研究对12周龄雄性小鼠在癫痫持续状态（status epilepticus, SE）造模3周后开展行为学评估，涉及三组小鼠：生理盐水注射假手术组（SHAM）、癫痫持续状态模型组（SE）以及癫痫持续状态+VH-N412给药组（SE + VH-N412），以检测其海马依赖性学习与记忆能力。基于嗅觉管道迷宫的行为学评估流程参照本团队此前发表的方法（Roman等, 2002; Girard等, 2014）执行；本研究使用的FVB/N小鼠品系在该任务中表现优异，相关结果已发表（Girard等, 2016）。简言之，实验小鼠先接受气味-奖赏关联训练：预先进行饮水限制的小鼠在测试箱（testing chambers, TC）中接受气味刺激。来自两个不同臂端的两种合成气味（草莓味与茉莉花香）被随机分配至对应臂端，并同时呈现给小鼠。两种气味分别被随机绑定为奖赏（一滴清水）或非厌恶性但令人不适的声音，奖惩规则随小鼠选择的臂端而定。[...]，# 嗅觉管道迷宫与旷场行为原始数据
[https://doi.org/10.5061/dryad.6wwpzgn8k]

## 数据集与文件结构说明
癫痫患者与动物模型均存在学习与记忆障碍，本研究旨在探究本团队开发的低温诱导分子VH-N412通过减轻神经退行性变与炎症反应，是否能够改善癫痫模型动物的记忆能力。癫痫持续状态造模3周后，对小鼠开展行为学测试：采用嗅觉管道迷宫与试间隔（inter-trial interval, ITI）范式评估其海马依赖性学习与记忆能力；通过旷场实验（open-field paradigm）检测相同小鼠的探索行为与自发运动活性，记录不同组小鼠的平均移动距离。

### 文件与变量说明
#### 文件：Behavior_RAW_DATA_OTM_and_OPEN_FIELD.xlsx
**描述：** 用于评估[...]