Zinc-induced pancreatic pathology in Zip5 Panc KO mice, Zip5 Total KO and control mice.

Zip5 recombination in pancreatic acinar cells was induced by activation of CRE:ERT2 expressed from an ela-Cre-ERT2 transgene and Zip5 recombination in all cells was created using a ubiquitously expressed CRE recombinase. Two to three weeks after weaning, control (Control), pancreas-specific Zip5-knockout (Zip5 Panc KO) and Zip5-total knockout mice (Zip5 Total KO) were given an I.P. injection of zinc sulfate (12.5 mg zinc/kg body weight) and 48 h later pancreata were harvested and paraffin sections were prepared, stained with hematoxylin and eosin and evaluated for the presence of zinc-induced vacuoles in acinar cells and for acinar cell atrophy. Sections were evaluated by the authors in this table. Each row represents an individual animal.