Zinc-induced pancreatic pathology in Zip5 Panc KO mice, Zip5 Total KO and control mice.

Zip5 recombination in pancreatic acinar cells was induced by activation of CRE:ERT2 expressed from an ela-Cre-ERT2 transgene and Zip5 recombination in all cells was created using a ubiquitously expressed CRE recombinase. Two to three weeks after weaning, control (Control), pancreas-specific Zip5-knockout (Zip5 Panc KO) and Zip5-total knockout mice (Zip5 Total KO) were given an I.P. injection of zinc sulfate (12.5 mg zinc/kg body weight) and 48 h later pancreata were harvested and paraffin sections were prepared, stained with hematoxylin and eosin and evaluated for the presence of zinc-induced vacuoles in acinar cells and for acinar cell atrophy. Sections were evaluated by the authors in this table. Each row represents an individual animal.胰腺腺泡细胞中的Zip5重组，可通过由ela-Cre-ERT2转基因（ela-Cre-ERT2 transgene）表达的CRE:ERT2的激活诱导获得；而全细胞Zip5重组则通过广泛表达的CRE重组酶（CRE recombinase）实现。仔鼠断乳后2至3周，对对照组（Control）、胰腺特异性Zip5敲除（Zip5 Panc KO）小鼠及全身Zip5敲除（Zip5 Total KO）小鼠进行硫酸锌的腹腔（I.P.）注射，注射剂量为12.5 mg锌/千克体重。注射后48小时收获胰腺，制备石蜡切片，经苏木精-伊红染色后，评估腺泡细胞中锌诱导空泡的存在情况及腺泡细胞萎缩程度。本表格中的所有切片均由本文作者完成评估，表格每一行对应一只实验动物。