DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 37.

DNA sequence of the gp63 fragment encoding the functional domain of leishmanolysin in natural isolates of Leishmania braziliensis from Corte de Pedra, Northeast Brazil: allele 37. The stretch of gp 63 previously described as encoding the functional domain of the molecule was amplified by PCR, cloned into pcr 2.1 vector and bidirectionally sequenced. Sequences from a panel of 41 parasite isolates were aligned and 45 alleles of the fragment could be identified based on indel and SNPs presented among cloned domains. Haplotypes of polymorphisms (indel and SNPs) that could be detected in at least two clones of the gp63 domain in a single isolate, and in at least two parasite isolates in the collection were considered alleles. Corte de Pedra is a region highly endemic for American tegumentary leishmaniasis (ATL) due to L. braziliensis. Several forms of ATL occur and close to 1,500 new cases are diagnosed in the region each year.采自巴西东北部科特德佩德拉（Corte de Pedra）地区的巴西利亚利什曼原虫（Leishmania braziliensis）自然分离株中，编码利什曼蛋白酶（leishmanolysin）功能结构域的gp63片段DNA序列：等位基因37。此前被报道可编码该分子功能结构域的gp63片段区域，通过聚合酶链式反应（PCR）扩增后，被克隆至pcr2.1载体，并完成双向测序。对41株利什曼原虫分离株的序列进行比对后，基于克隆结构域间存在的插入缺失（indel）与单核苷酸多态性（SNPs），可鉴定出该片段的45种等位基因。在单株分离株的gp63结构域至少两个克隆中，以及在本次收集的至少两株利什曼原虫分离株中均可检测到的多态性（插入缺失与单核苷酸多态性）单倍型，被界定为等位基因。科特德佩德拉是巴西利亚利什曼原虫（L. braziliensis）所致美国皮肤利什曼病（ATL）的高流行地区。该地区存在多种临床表型的美国皮肤利什曼病，每年新增确诊病例近1500例。