Expression data of Gr-MDSCs under different treatment. Expression data of Gr-MDSCs under different treatment

Myeloid derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells with differentiation program partially blocked in pathological conditions such as cancer. Through inhibition of T cells especially cytotoxic T lymphocytes (CTLs), MDSCs play important tumor-promoting roles and maintain a state of immunological anergy and tolerance We used microarrays to profile gene expression of Gr-MDSC and identified differentially altered genes under different drug treatment. Overall design: For 8-12 weeks old PB-Cre+ PtenL/L p53L/L Smad4L/L mTmGL/+ LSL-LucL/+ (CPPSML) male mice, once the prostate tumor reached 150mm3, the mice were surgically castrated and switched to enzalutamide-admixed diet for 4 weeks. The mice were then euthanized for isolating Gr-MDSCs from prostate tumors. Gr-MDSCs were then seeded into well-plate and treated with Dactolisib, Dasatinib or MDSC as control. RNA was isolated after 6h treatment and prepared for microarray.