Transcriptomic analysis of lung tissue from WT mice treated with or without MSDC-0602K following influenza virus infection

To identify molecular characteristics of lung tissue from WT mice treated with DMSO or a new insulin sensitizer MSDC-0602K at day 4 post influenza virus infection, we isolated RNA from lungs with various treatments and examined by bulk RNA-seq. We found a large number of gene profiles were altered following MSDC-0602K treatment in lungs. Interestingly, gene sets involved inflammatory responses were enriched in DMSO treatd mouse lungs whereas gene set of fatty acid metabolism was enriched in MSDC-0602K treated mouse lung, suggesting MSDC-0602K treatment diminshed pulmonary inflammation and altered metabolic status during influenza virus infection. Influenza virus infected-WT mice were administrated with DMSO or MSDC-0602K daily from day 1 to day 3 post viral infection. Lungs were obtained at day 4 post influenza virus infection for sequencing.