Impaired antigen-specific memory B cell and plasma cell responses including lack of specific IgG upon SARS-CoV-2 BNT162b2 vaccination among Kidney Transplant and Dialysis patients

Single cell transcriptome, cell-surface proterin, TCR and BCR sequencing of FACS-sorted activated T and B cells of the peripheral blood of patients vaccinated against COVID19 after kidney transplant transplantation Overall design: Sequencing of RNA, TCR and BCR was performed from FACS-sorted activated T cells (CD38+ HLA-DR+), memory B cells (CD38low CD27+) and plasmabolasts (CD38high CD27high) from the peripheral blood of four patients vaccinated against COVID19 after kidney transplantation, using the 10X Genomics platform with the Single Cell 5' Library & Gel Bead Kit