DMXL1 interaction proteomics from U2OS cells infected with SidK

To assess the effect of SidK expression on DMXL1 interaction with the V1 subunits of the V-ATPase, U2OS cells stably expressing DMXL1 tagged with mNeonGreen (mNG) were infected with lentivirus to express either mCherry or mCherry-SidK. Post-infection, cells were harvested to perform immunoprecipitation (IP) of DMXL1-mNG using magnetic beads coupled with a nanobody against mNG. IP samples were subsequently processed for TMT-MS analysis. As negative control, immunoprecipitation was also performed from parental U2OS cells expressing mCherry. Each condition was performed in triplicate.