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Metagenomic Dataset of a Magpie-lark (Grallina cyanoleuca)

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Mendeley Data2021-04-06 更新2026-04-09 收录
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A cutaneous tissue sample from a wild mudlark (Grallina cyanoleuca) was collected at Canley Heights Veterinary Clinic, NSW-2166 (Sample ID: 19-1000) and was deposited at Charles Sturt University. The genomic DNA was isolated according to methods described previously using a ReliaPrep gDNA Tissue Miniprep System (Promega, USA). The library was prepared using the QIAseq FX DNA Library Kit (Qiagen) and sequenced with the sequencing reads the length of 150-bp paired-end on Illumina® NextSeq 500 platform through the Australian Genome Research Facility.

本研究于新南威尔士州2166邮编的康利高地兽医诊所采集了一只野生灰背钟鹊(Grallina cyanoleuca)的皮肤组织样本,样本编号为19-1000,且该样本已保藏于查尔斯特大学(Charles Sturt University)。按照既往报道的方法,使用ReliaPrep gDNA组织微量制备系统(Promega,美国)提取基因组DNA。采用QIAseq FX DNA文库制备试剂盒(Qiagen)构建测序文库,并通过澳大利亚基因组研究设施(Australian Genome Research Facility)在Illumina® NextSeq 500测序平台上开展读长为150 bp的双端测序。
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2021-04-06
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