Stanol Data From Sediment and Catchment Soil Samples in Chisholm Lake, Alaska, 2021-2023

This dataset contains concentrations of stanols analysed by Gas chromatography/mass spectrometry (GC/MS) from Chisholm lake, Alaska, USA (64.302268, -146.68733) sediment and catchment soil samples. This study aimed to improve understanding of long-term human impacts to this sub-arctic watershed and lake system by generating proxy records of human presence using fecal sterol analysis. The fecal sterol record spans the last ~2,500 years, facilitating comparisons with both historical and archaeological records from the region. Fecal sterols were extracted following the protocol outlined by Mackay et al. (2020; reference provided in methodology). Briefly, this involved extraction from the soils and sediments using microwave assisted extraction, saponification, Solid Phase Extraction (SPE) and silica gel column separation, methylation and GC/MS analysis. The research took place in 2021-2023.

本数据集收录了采自美国阿拉斯加州奇泽姆湖（Chisholm Lake，地理坐标：64.302268, -146.68733）沉积物及集水区土壤样本中的甾烷醇（stanols）浓度数据，该浓度经气相色谱-质谱联用法（Gas chromatography/mass spectrometry，GC/MS）测定得到。本研究旨在通过粪便甾醇分析构建人类活动的代用指标记录，以深化对该亚北极流域与湖泊系统所受长期人类活动影响的认知。该粪便甾醇记录覆盖了近2500年的时间跨度，可辅助开展与该区域历史及考古记录的对比研究。粪便甾醇的提取流程参照了Mackay等人（2020年；完整参考文献见研究方法部分）所提出的实验方案。简言之，该流程包括微波辅助萃取、皂化反应、固相萃取（Solid Phase Extraction，SPE）、硅胶柱层析分离、甲基化衍生以及气相色谱-质谱联用法分析等步骤，用于提取土壤与沉积物中的粪便甾醇。本研究于2021至2023年间开展。