Additional file 1: of A new mechanism of trastuzumab resistance in gastric cancer: MACC1 promotes the Warburg effect via activation of the PI3K/AKT signaling pathway

Figures S1 to S6. Figure S1: The expression of proteins in GC cells and the sequences of ectopic MACC1 and shRNA. Figure S2: The combination of trastuzumab and glycolysis inhibitors synergisticly inhibit glycolysis in HER2 positive GC cells. Figure S3: The combination of trastuzumab and glycolysis inhibitors synergisticly inhibit glycolysis in HER2 positive GC cells. Figure S4: MACC1 enhanced the Warburg effect in vivo. Figure S5: Combination of trastuzumab and oxamate effectively inhibited the Warburg effect in vivo. Figure S6: The apoptosis of indicated cells after treated with Ttzm. (ZIP 38363Â kb)补充图S1至S6。 图S1：胃癌细胞中的蛋白表达情况，以及异位MACC1与短发夹RNA（short hairpin RNA, shRNA）的序列。 图S2：曲妥珠单抗（trastuzumab）与糖酵解抑制剂联合使用，可协同抑制HER2阳性胃癌细胞的糖酵解。 图S3：曲妥珠单抗（trastuzumab）与糖酵解抑制剂联合使用，可协同抑制HER2阳性胃癌细胞的糖酵解。 图S4：MACC1在体内增强瓦博格效应（Warburg effect）。 图S5：曲妥珠单抗（trastuzumab）与草氨酸盐（oxamate）联合使用，可在体内有效抑制瓦博格效应（Warburg effect）。 图S6：经Ttzm处理后的指定细胞的凋亡情况。（ZIP 38363 KB）