Rodent pathogen status, hantavirus (DP1.10064.001)

Small mammal sampling is based on the lunar calendar, with timing of sampling constrained to occur within 10 days before or after the new moon. Typically, core sites are sampled 6 times per year, and relocatable sites 4 times per year. Small mammals are sampled using box traps (models LFA, XLK, H.B. Sherman Traps, Inc., Tallahassee, FL, USA). Box traps are arrayed in three to eight (depending on the size of the site) 10 x 10 grids with 10m spacing between traps at all sites. Blood samples for pathogen testing are collected only once per individual per sampling bout, but an individual may be resampled in subsequent bouts. Rodent-borne pathogen status data are typically only collected on three of the mammal trapping grids at each site, with these grids designated as 'pathogen grids'. Pathogen grids are trapped for three consecutive (or nearly) nights per sampling event to facilitate the generation of robust density estimates. The remaining grids, 'diversity grids' are only sampled for one night per sampling event. Once a grid has been designated as a pathogen grid (after an initial assessment period of two years), that classification will apply for all subsequent trapping seasons for consistent, long-term data collection.

小型兽类采样基于农历，采样时段需严格限定在新月前后10天以内。通常情况下，核心样地每年开展6次采样，可移动样地每年开展4次采样。小型兽类采样采用盒式诱捕器（型号为LFA、XLK，制造商为美国佛罗里达州塔拉哈西市H.B. Sherman诱捕器公司）。所有样地的盒式诱捕器均按3至8组（依样地面积而定）10×10的网格阵列布设，各诱捕器间距统一为10米。用于病原体检测的血液样本，在单次采样周期内仅对每个个体采集一次，但后续采样周期中可对该个体进行再次采样。啮齿动物携带病原体的状态数据，通常仅在每个样地的3个兽类诱捕网格上收集，这类网格被划定为“病原体监测网格”。每个采样事件中，病原体监测网格需连续（或近乎连续）布设3晚诱捕器，以获取稳健可靠的种群密度估计值。剩余的网格即“多样性监测网格”，每个采样事件仅布设一晚进行采样。某网格一旦被指定为病原体监测网格（需经过为期两年的初始评估期），该分类将适用于所有后续的诱捕季，以保障长期数据收集的一致性与延续性。