Table 3 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Table 3 Concentrations of identified jasmonic acid (JA)-related compounds in the cotyledon, radicle, and seed coat of developing Phaseolus vulgaris seeds. TissueWeight (mg FW)αLA (ng mg 1 FW)OPDA (ng mg 1 FW)OPC-8:0 (ng mg 1JA (ng mg 1FW)FW)Cotyledon187.8 ±4.11 ±0.33 ±0.24 ±0.05 ±9.701.86a0.19a0.15a0.02aRadicle3.2 ± 0.168.19 ± 14.12b1.67 ± 0.53 a4.12 ± 0.56b0.21 ± 0.06 aSeed coat94.8 ± 5.242.44 ± 08.86b15.88 ± 1.22b2.70 ± 0.01b0.53 ± 0.21 a αLA, α- linolenic acid; OPDA, 12-oxo-phytodienoic acid; OPC-8, 3 -oxo-2-(2-(Z)- pentenyl) cyclopentane-1-octanoic acid. These JA-related compounds in each tissue extract prepared from three different developing P. vulgaris seeds were quantified by LC-ESI-MS analysis. Concentrations of the JA-related compounds in each tissue are shown per milligram of fresh weight (FW). Each value represents the mean ± standard deviation (n = 3). Lowercase superscripted letter indicates significant differences (p <0.05) between the tissue types for each JArelated compound.表3 菜豆（*Phaseolus vulgaris*）发育种子的子叶、胚根与种皮中鉴定得到的茉莉酸（jasmonic acid, JA）类化合物浓度 | 组织 | 鲜重（mg FW） | α-亚麻酸（αLA, ng·mg⁻¹ FW） | 12-氧-植物二烯酸（OPDA, ng·mg⁻¹ FW） | 3-氧代-2-(2-(Z)-戊烯基)环戊烷-1-辛酸（OPC-8:0, ng·mg⁻¹ FW） | 茉莉酸（JA, ng·mg⁻¹ FW） | |------|--------------|------------------------------|--------------------------------------|-----------------------------------------------------------|--------------------------| | 子叶 | 187.8 ± 4.1 | 1.86 ± 0.33^a | 0.19 ± 0.15^a | 0.02 ± 0.05^a | 9.70 | | 胚根 | 3.2 ± 0.1 | 68.19 ± 14.12^b | 1.67 ± 0.53^a | 4.12 ± 0.56^b | 0.21 ± 0.06^a | | 种皮 | 94.8 ± 5.2 | 42.44 ± 8.86^b | 15.88 ± 1.22^b | 2.70 ± 0.01^b | 0.53 ± 0.21^a | 注：αLA即α-亚麻酸（α-linolenic acid）；OPDA即12-氧-植物二烯酸（12-oxo-phytodienoic acid）；OPC-8:0即3-氧代-2-(2-(Z)-戊烯基)环戊烷-1-辛酸（3-oxo-2-(2-(Z)-pentenyl) cyclopentane-1-octanoic acid）。本研究采用液相色谱-电喷雾电离质谱（LC-ESI-MS）分析法，对从3份不同发育时期的菜豆种子中制备的各组织提取物中的上述茉莉酸类化合物进行定量检测。各组织中茉莉酸类化合物的浓度以每毫克鲜重（FW）计。所有数值均以平均值±标准差表示（n=3）。小写上标字母代表同一茉莉酸类化合物在不同组织间存在显著差异（p<0.05）。