Quantification of the light microscopic analyzes after anti-tyrosine hydroxylase (TH) staining of the olfactory bulb (OB) and the striatal fibers.

No significant differences were seen 2, 5 and 20 days after intranasal application of 0.5(p>0.05, n = 3, striatal fibers were analyzed in a field of 45×45 µm, TH positive neurons were manually counted in the glomerular layer of the OB (total magnification 200×)). Error bars represent SEM.经鼻腔给予0.5后，于给药后第2、5及20天未观察到显著差异（p>0.05，n=3；实验中于45×45 µm的视野内分析纹状体纤维，并在总放大倍数200×的条件下，手动计数嗅球（Olfactory Bulb, OB）小球层内的酪氨酸羟化酶（Tyrosine Hydroxylase, TH）阳性神经元）。误差棒代表标准误（Standard Error of the Mean, SEM）。