Theta-phase-specific modulation of dentate gyrus memory neurons

The theta rhythm, a quasi-periodic 4-10 Hz oscillation, is observed during memory processing in the hippocampus, with different phases of theta hypothesized to separate independent streams of information related to the encoding and recall of memories. At the cellular level, the discovery of hippocampal memory cells (engram neurons), as well as the modulation of memory recall through optogenetic activation of these cells, has provided evidence that certain memories are stored, in part, in a sparse ensemble of neurons in the hippocampus. In previous research, however, engram reactivation has been carried out using open loop stimulation at fixed frequencies; the relationship between engram neuron reactivation and ongoing network oscillations has not been taken into consideration. To address this concern, we implemented a closed-loop reactivation of engram neurons that enabled phase-specific stimulation relative to theta oscillations in the local field potential. Using this real-time approa..., This dataset is composed of both freezing and LFP data from mice undergoing optogentic stimulation. The mice had bilateral fiber optics implanted over DG as well as a recording electrode in CA1. The files have the motion of the animal, the stimulation times, and the raw LFP values., The files are .csv, so almost any software can open the data files.Â

θ节律（theta rhythm）是一种准周期性的4-10 Hz脑电振荡，在海马体的记忆加工过程中可被观测到，学界假说认为θ节律的不同相位可分离与记忆编码、提取相关的独立信息流。在细胞层面，海马记忆细胞（印迹神经元(engram neurons)）的发现，以及通过光遗传激活这些细胞对记忆提取进行的调控，为部分记忆存储于海马体中稀疏神经元集群内提供了实验证据。但在既往研究中，印迹神经元的再激活均采用固定频率的开环刺激范式，并未考虑印迹神经元再激活与进行中的网络振荡之间的关联。为解决这一问题，我们开发了针对印迹神经元的闭环再激活方案，可基于局部场电位中的θ节律实现相位特异性刺激。本研究采用的实时方法……本数据集包含接受光遗传刺激的小鼠的僵冻行为数据与局部场电位（LFP）数据。实验小鼠的齿状回（DG）区域植入了双侧光纤套管，同时在海马CA1区埋置了记录电极。数据文件包含动物运动信息、刺激时刻与原始局部场电位数值。所有数据文件均为逗号分隔值（.csv）格式，几乎可通过任意软件打开。