RNA-seq analysis for mRNA expression change in oxygen/glucose deprivation-treated cultured oligodendrocyte precursor cells

Oligodendrocyte precursor cells (OPCs) regulate neuronal, glial and vascular systems in diverse ways and display phenotypic heterogeneity beyond their well-known role as a reservoir for mature oligodendrocytes. Here we demonstrated that perivascular OPCs in cerebral cortex were increased coupling with post-stroke angiogenesis in a mouse model of stroke induced by middle cerebral artery occlusion (MCAO). RNA-seq analysis revealed that primary cultured OPCs notably increased the gene expressions of numerous pro-angiogenic factors, while decreased those related to OPC maturation after oxygen glucose deprivation. The data showed that cerebral ischemia shifts the phenotype of OPCs to perivascular subtypes which can promote angiogenesis. Overall design: mRNA profiles of primary cultured rat oligodendrocyte precursor cells incubated under normal or oxygen glucose deprivation conditions for 6 hours were generated by deep sequencing, in quadruplicate, using Illumina MiSeq.