Spatiotemporal development of the human T follicular helper cell response to Influenza vaccination

<strong>The code that accompanies these data is available on GitHub.</strong> <strong>Data</strong> <strong>The two rds-format (R Data Serialization) files contain Seurat objects used for analyses.</strong> <strong>intergrated_Tcells_harmony_bydonor.rds contains all T cells.</strong> <strong>intergrated_Tfh_harmony_bydonor.rds is a subset of the previous file containing the TFH and Treg lineages.</strong> <strong>Set2_10x_clones.tsv is paired TCR information for cells from the 10X experiment for all cells in intergrated_Tcells_harmony_bydonor.</strong> <strong>TwoYear_LN_Bcell_freq_time.csv contains the B cell subset frequencies in the lymph node by time point.</strong> <strong>Ali_paired_sc_clones_JCC283.tsv contains paired TCR sequences of cTFH and LN TFH cells obtained by single-cell sorting and target PCR. </strong> <strong>Ali_bulk_allDonor_clones.tsv contains bulk TCR alpha and beta chains of sorted cTFH and LN TFH generated using a 5'-RACE protocol.</strong>本数据集配套代码已公开于GitHub平台。 数据集文件 本次提供的两个rds格式（R数据序列化格式，R Data Serialization）文件均为用于分析的Seurat对象。 文件intergrated_Tcells_harmony_bydonor.rds包含所有T细胞数据。 文件intergrated_Tfh_harmony_bydonor.rds为上述文件的子集，仅包含滤泡辅助性T细胞（TFH, Follicular Helper T Cell）与调节性T细胞（Treg, Regulatory T Cell）谱系数据。 Set2_10x_clones.tsv文件包含intergrated_Tcells_harmony_bydonor.rds中所有10X单细胞测序实验来源细胞的配对T细胞受体（TCR, T-cell Receptor）信息。 TwoYear_LN_Bcell_freq_time.csv文件包含不同时间点淋巴结内B细胞（B Cell）亚群的占比数据。 Ali_paired_sc_clones_JCC283.tsv文件包含通过单细胞分选与靶向PCR技术获取的循环滤泡辅助性T细胞（cTFH, circulating T Follicular Helper Cell）与淋巴结滤泡辅助性T细胞（LN TFH, Lymph Node TFH）的配对TCR序列数据。 Ali_bulk_allDonor_clones.tsv文件包含通过5'-末端快速扩增技术（5'-RACE, 5'-Rapid Amplification of cDNA Ends）扩增得到的分选后cTFH与LN TFH细胞的批量T细胞受体α链与β链序列数据。