Data from: Combined low-frequency ultrasound and urokinase-containing microbubbles in treatment of femoral artery thrombosis in a rabbit model

This paper aims to study the thrombolytic effect of low-frequency ultrasound combined with targeted urokinase-containing microbubble contrast agents on treatment of thrombosis in rabbit femoral artery; and to determine the optimal combination of parameters for achieving thrombolysis in this model. A biotinylated-avidin method was used to prepare microbubble contrast agents carrying urokinase and Arg-Gly-Asp-Ser (RGDS) peptides. Following femoral artery thrombosis in New Zealand white rabbits, microbubble contrast agents were injected intravenously, and ultrasonic exposure was applied. A 3 × 2 × 2 factorial table was applied to categorize the experimental animals based on different levels of combination of ultrasonic frequencies (Factor A: 1.6 MHz, 2.2 MHz, 2.8 MHz), doses of urokinase (Factor B: 90,000 IU/Kg, 180,000 IU/Kg) and ultrasound exposure time (Factor C: 30 min, 60 min). A total of 72 experimental animals were randomly divided into 12 groups (n = 6/group). Doppler techniques were used to assess blood flow in the distal end of the thrombotic femoral artery during the 120 minutes thrombolysis experiment. The rate of recanalization following thrombolysis was calculated, and thrombolytic efficacy was evaluated and compared. The thrombolytic recanalization rate for all experimental subjects after thrombolytic therapy was 68.1%. The optimal parameters for thrombolysis were determined to be 1) an ultrasound frequency of 2.2 MHz and 2) a 90,000 IU/kg dose of urokinase. Ultrasound exposure time (30 min vs. 60 min) had no significant effect on the thrombolytic effects. The combination of local low-frequency ultrasound radiation, targeted microbubbles, and thrombolytic urokinase induced thrombolysis of femoral artery thrombosis in a rabbit model. The ultrasonic frequency of 2.2 MHz and urokinase dose of 90,000 IU/kg induced optimal thrombolytic effects, while the application of either 30 min or 60 min of ultrasound exposure had similar effects.

本研究旨在探讨低频超声联合靶向携带尿激酶的微泡造影剂对新西兰白兔股动脉血栓的溶栓效果，并确定该动物模型中实现溶栓的最优参数组合。采用生物素-亲和素法制备携带尿激酶与精氨酸-甘氨酸-天冬氨酸-丝氨酸（Arg-Gly-Asp-Ser, RGDS）肽的微泡造影剂。对新西兰白兔构建股动脉血栓模型后，经静脉注射微泡造影剂并实施超声辐照。本研究采用3×2×2析因设计对实验动物进行分组，分组因素包括超声频率（因素A：1.6 MHz、2.2 MHz、2.8 MHz）、尿激酶剂量（因素B：90000 IU/kg、180000 IU/kg）以及超声辐照时长（因素C：30 min、60 min）。共计72只实验动物被随机分为12组，每组6只。在120分钟的溶栓实验过程中，采用多普勒技术检测血栓远端股动脉的血流情况。计算溶栓后的血管再通率，并对溶栓疗效进行评估与比较。所有实验动物经溶栓治疗后的血管再通率为68.1%。本研究确定的最优溶栓参数为：1）超声频率2.2 MHz；2）尿激酶剂量90000 IU/kg。超声辐照时长（30 min与60 min）对溶栓效果无显著影响。局部低频超声辐照、靶向微泡与溶栓性尿激酶联合使用，可诱导兔股动脉血栓模型的血栓溶解。其中2.2 MHz超声频率联合90000 IU/kg尿激酶剂量可取得最优溶栓效果，而30 min与60 min的超声辐照时长疗效相近。