C57Bl/6J Mouse Hippocampus Ca1 Pyramidal Cell Morphologies

title : Reconstruction of hippocampus CA1 cell morphologies specimen : Mus musculus sex : male strain : C57BL/6J age : post-natal day 13-16 This neuron was recorded and filled with biocytin (3 mg/ml) in a 300 µm thick coronal slice of rat hippocampus, using 2 - 10 MOhm patch pipettes. 3,3′-diaminobenzidine (DAB) was used for revelation. The slice was fixed and the cell reconstructed with Neurolucida using a 100x oil immersion objective.标题：海马CA1区细胞形态重建 标本：小家鼠（Mus musculus） 性别：雄性 品系：C57BL/6J 年龄：出生后第13至16天 本神经元于300 µm厚的大鼠海马冠状脑片内完成记录，并以浓度为3 mg/ml的生物胞素（biocytin）进行胞内填充，实验采用电阻为2~10 MΩ的膜片钳电极。通过3,3′-二氨基联苯胺（3,3′-diaminobenzidine，DAB）完成显色显影步骤。随后对脑片进行固定处理，并借助100倍油浸物镜，通过Neurolucida软件完成该细胞的形态重建。