Detection of recombinant PVY CP and CP mutants by DAS-ELISA and TAS-ELISA.

aRecombinant wild-type or mutated CPs of PVYO-UK and PVYN-605 were expressed in E. coli, and the bacterial cell lysate was used for detection of CP. Total protein from E. coli expressing no foreign protein and virions of PVYO-UK and PVYNTN Nevski purified from tobacco plants were used as controls. Absorbance at 405 nm (A405) was recorded using a microtiter plate reader. The sample was regarded as positive (+) if the value was two times higher (± standard deviation) than that of the total protein from E. coli expressing no foreign protein used as a negative (−) control. Detection of recombinant PVY CP and CP mutants by DAS-ELISA and TAS-ELISA.