In vivo validation of predicted fitness effects at single-base resolution in a Brachypodium distachyon mutant population

Datasets used in the analyses of the SIEVE population in the article 'In vivo validation of predicted fitness effects at single-base resolution in a Brachypodium distachyon mutant population'. snps.combined.vcf.gz: genotypic data for point mutations in the SIEVE population at M2 and M5 generations. scores.zip: scores used to predict the effect of point mutations in burden and purging tests: SIFT, ESM, PlantCAD, a2z, and PhytoExpr predictions gene_annotations.zip: gene annotations used to classify genes in gene depletion analyses: GO classes and PMN pathways SIFT_DB.zip: SIFT4G database used to annotate missense variants (consequences on protein sequence, in addition to SIFT scores) phenotypes.zip: phenotypic data in the SIEVE population at M3 and M4 generations: germination rate (germination), heading date (heading), plant height (height), and total seed weight (weight)本文《二穗短柄草（Brachypodium distachyon）突变体群体中单碱基分辨率预测适合度效应的体内验证》中，用于SIEVE群体分析的数据集如下： snps.combined.vcf.gz：SIEVE群体M2代与M5代点突变的基因型数据。 scores.zip：用于负荷测试与清除测试中点突变效应预测的评分数据集，包含SIFT、ESM、PlantCAD、a2z及PhytoExpr的预测结果。 gene_annotations.zip：用于基因耗竭分析中基因分类的基因注释数据集，包含GO（Gene Ontology）功能类与PMN通路。 SIFT_DB.zip：用于错义变异注释的SIFT4G数据库（除SIFT评分外，还涵盖蛋白质序列影响相关信息）。 phenotypes.zip：SIEVE群体M3代与M4代的表型数据，包含发芽率（germination）、抽穗期（heading）、株高（height）及总种子重量（weight）。