Intestinal epithelial GR in response to DEX treatment. Intestinal epithelial GR in response to DEX treatment

The goal of this project is to investigate the role of glucocorticoids and their receptor, glucocorticoid receptor (GR) , in intestinal stress response and tissue homeostasis.We generated a mouse model with specific deletion of GR in intestinal epithelium (GR iKO mice) and examined colonic transcriptomes of adrenalectomized (ADX) Flox control and GR iKO mice in response to dexamethasone (DEX) treatment by microarray analysis. Overall design: Total RNA were extracted from colon tissues of 4 pairs adrenalectomized (ADX) Flox control and GR iKO mice treated with or without 1 mg/Kg dexamethasone (DEX) for 6 hours. Gene expression profiles were analyzed using Agilent Whole Mouse Genome 4x44 multiplex format oligo arrays (014868) (Agilent Technologies, Santa Clara, CA), following the Agilent 1-color microarray-based gene expression analysis protocol.