Inducible degradation of Sros1 promotes IFN-γ-activated macrophage innate response by stabilizing STAT1 mRNA

To identify new target genes of miR-1, the affinity based purification experiments of mouse mRNAs using biotin-labeled miR-1 as probes was performed in peritoneal macorphages. Enriched RNAs and input samples were then subjected to RNA sequencing using an Illumina platform. To de novo explore miR-1 target genes in peritnoeal macrophage cells, we performed a modified miRIP protocol using biotin-labeled miR-1 as oligo nucleotide probes to in vivo affinity purify the genes using nucleic acid hybridization and use Illumina Next Generation Sequencing assay to identify the associated RNAs.