COS7 CHMP4A+VPS4DN
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Tilt Series Date: 2011-12-22 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: imod Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine Sample Preparation: manual blotting
倾斜序列日期：2011-12-22 数据采集者：科拉·伍德沃德（Cora Woodward） 物种/标本：非洲绿猴（Cercopithecus aethiops） 细胞株：COS-7 ATCC CRL-1651 倾斜序列设置：单轴倾斜，倾斜范围为(-60.0°, 60.0°)，步长1°，采用恒定角度增量；电子辐照剂量为150.0 eV/Ų，散焦值为-6.0 μm，放大倍数为22500× 采集软件：UCSF tomo 上传方式：rundir 所用处理软件：imod 合作者及分工：构建体由韦斯·桑德奎斯特（Wes Sundquist）提供；细胞转染、冷冻及成像工作由科拉·伍德沃德在加州理工学院（Caltech）完成 纯化/培养条件及处理方式：全细胞于载网上培养，并使用Lipofectamine（脂质转染试剂）进行转染 样品制备：手动印迹
