Data from: Identification, in silico characterization and expression analysis of Deschampsia antarctica DaDREB2A transcription factor gene involved in abiotic stress responses

The dataset contains the annotated nucleotide sequences of two predicted allele variants of DaDREB2A transcription factor gene of Deschampsia antarctica (Poaceae), as well as the alignment of these sequences and two DaDREB2A gene allele variants that were cloned and sequenced from a D. antarctica plant (NCBI GenBank accession numbers PX861127 and PX963160). It also contains the alignment of the corresponding DaDREB2A protein variants.The DaDREB2A variants were assembled using D. antarctica raw genomic and transcriptomic sequencing data from the NCBI Sequence Read Archive (SRA; https://www.ncbi.nlm.nih.gov/sra/). DREB2A-like gene sequence from Lolium arundinaceum (AJ717396.1), a species phylogenetically closely related to Deschampsia, was retrieved from NCBI GenBank and used as reference to query the D. antarctica genomic nanopore datasets (SRX24981177–SRX24981180, SRX24937479) using discontiguous MegaBLAST with the default parameters. Based on the identified long homologous regions, a consensus gene sequence was constructed and subsequently refined using higher-accuracy short-read genomic and transcriptomic data (SRX465632, SRX5305101–SRX5305108). Sequence assembly and manual curation were performed using MEGA11 software. The positions of exons and mRNA were predicted by comparing genomic and transcriptomic data.本数据集包含南极发草（Deschampsia antarctica，禾本科Poaceae）DaDREB2A转录因子基因的2个预测等位变异的注释核苷酸序列，同时包含上述序列与从1株南极发草中克隆测序得到的2个DaDREB2A基因等位变异（NCBI GenBank登录号PX861127、PX963160）的序列比对结果，以及对应的DaDREB2A蛋白变异体的序列比对。 上述DaDREB2A变异体基于NCBI序列读取存档（Sequence Read Archive, SRA; https://www.ncbi.nlm.nih.gov/sra/）中的南极发草原始基因组和转录组测序数据组装得到。从NCBI GenBank获取与发草属系统发育关系密切的苇状羊茅（Lolium arundinaceum）的DREB2A类基因序列（登录号AJ717396.1）作为参考，使用默认参数的不连续MegabLAST（discontiguous MegaBLAST）检索南极发草基因组纳米孔测序数据集（SRX24981177–SRX24981180、SRX24937479）。基于鉴定得到的长同源区域，构建共识基因序列，随后利用高精度短读长基因组和转录组数据（SRX465632、SRX5305101–SRX5305108）进行序列优化。序列组装与人工校验工作采用MEGA11软件完成。通过比对基因组与转录组数据，预测外显子及mRNA的位置。