Astrovirus replication is dependent on induction of double membrane vesicles through a PI3K-dependent, LC3-independent pathway

The cellular response to astrovirus infection is not well defined. We used single cell RNA sequencing (scRNA-seq) to determine cellular response to astrovirus early or late in infection. Overall design: Caco-2 cells were infected with HAstV-1 or mock control and collected at 4, 8, and 24-hpi for scRNA-seq analysis