DBiT-seq sequencing data for mouse embryo

Title: High spatial resolution multi-omics atlas sequencing of mouse embryos Organism: Mus musculus Experiment type: Expression profiling by high throughput sequencing Summary: Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution. Overall design: Applying new approach – microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10μm, 25μm, 50μm).

标题：小鼠胚胎高空间分辨率多组学图谱测序
实验生物：小家鼠（Mus musculus）
实验类型：基于高通量测序的表达谱分析
摘要：对组织切片中的mRNA与蛋白质实施微流控确定性条形码标记，结合高通量测序技术，可在基因组尺度上构建高空间分辨率多组学图谱。将该方法应用于小鼠胚胎组织，能够以单细胞层分辨率揭示早期器官发生阶段的主要组织（亚）类型、脑微血管系统以及视泡的精细结构。
整体实验设计：采用新型研究方法——用于空间组学测序的组织微流控确定性条形码技术，对分辨率为10μm、25μm、50μm的小鼠胚胎组织开展实验。