Water record of Magallana angulata F2 generation exposed to transgenerational ocean acidification during laboratory experiments in 2021/2022

The basic observed water characteristics (pH, temperature, salinity) of Magallana angulata (also known as Crassostrea angulata) F2 generation from larval stage to adult in the hatchery located in Zhanjiang, China (21.07°N, 110.74°E) for around one year. The F2 larvae were acquired by artificial fertilization of matured F1 generation (F1_8.1-7.4 and F1_7.4-8.1). Embryos from F1_8.1-7.4 were separated into two pH levels: F2_8.1-7.4-8.1 and F2_8.1-7.4-7.4. The embryos from F1_7.4-8.1 were separated into two pH levels: F2_7.4-8.1-8.1 and F2_7.4-8.1-7.4. There were four treatments for F2 generation and reared till 2022 to test the transgenerational OA effects on oyster biological responses. Low pH was driven by bubbling of CO2-enriched air mixed with ambient air into filter seawater in the treatment tanks. pH and temperature were monitored daily with pH meter (Mettler Toledo InLab®, Switzerland) calibrated with NBS standards. Salinity was monitored using a dual-scale refractometer (MingRui, China). The seawater was utterly changed every two days during larval stage and every week for juvenile stage.