Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project)

<p>Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with time points taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017].</p>

<p>See Niskin Bottle and Cast List EN584 to link specific casts and bottles to each experiment: <a href=\"https://www.bco-dmo.org/dataset/717427\" target=\"_blank\">https://www.bco-dmo.org/dataset/717427</a>.</p>

本研究采用TECAN Spectrafluor Plus型酶标仪（激发波长360 nm，发射波长460 nm），在24~48小时的孵育周期内检测荧光信号，每4~6小时采集一组时间点数据。水解速率通过孵育体系中荧光信号随时间的增长速率，结合一系列已知浓度的荧光团（fluorophore）标准品计算得到。用于计算水解速率及生成本文附图的代码脚本，可在关联的GitHub代码仓库[Hoarfrost, 2017]中获取。

可通过尼斯金采水器（Niskin Bottle）与EN584号测站清单，将各实验与对应的具体测站事件及采水器进行关联：<a href="https://www.bco-dmo.org/dataset/717427" target="_blank">https://www.bco-dmo.org/dataset/717427</a>。