RNA-seq gene expression of flow cytometry sorted breast cancer stem cell populations

MMTV-PyMT transgenic FVBN mice spontaneously developed breast tumors. Two tumors were collected as replicate samples, and cells from the dissociated tissue were sorted into 2 cancer stem cell (CSC) populations, along with the remaining non-CSCs. The samples were submitted for RNAseq to generate differential gene expression profiles of the breast tumor CSC populations. Gene expression profile of ALDH positive and CD29 hi, CD61 positive CSC populations compared to non-CSC from mouse breast tumor.