Diel Multi Omics from the Hawaii Diel Sampling (HaDS)

Diel Multi Omics From the Hawaii Diel Sampling (HaDS) Here, we present data from the Hawaiʻi Diel Sampling (HaDS) survey, which represents a high-resolution sampling of surface ocean microbial communities every 1.5 hours for 48 hours across two environments: Kāneʻohe Bay on the windward coast of Oʻahu, Hawaiʻi, and the adjacent offshore. At both the coastal Kāneʻohe Bay station (HP1) and the adjacent offshore station (STO1), we sampled at 33 time-points across 48 hours in August 2021, and subsequently produced 59 metatranscriptomes, 65 short-read metagenomes, 8 long-read metagenomes, and 66 transfer RNA transcript libraries, in addition to measurements of ocean biogeochemistry. We also generated four deeply-sequenced short-read metagenomes from samples collected in the late fall and spring prior to HaDS through routine Kāneʻohe Bay Time-series sampling (Tucker et al., 2021; Tucker et al., 2025). The goal of HaDS is to characterize microbial responses to diel changes in ocean biogeochemistry and investigate mechanisms of transcriptional and translation regulation. These data were collected by Dr. A. Murat Eren of the Helmholtz Institute for Functional Marine Biodiversity. Further information can be found here: https://merenlab.org/data/hads/ and in the forecoming data description paper (Tucker et al., in review). Timestamp is in UTC.夏威夷昼夜采样计划（HaDS）昼夜多组学数据集 本研究公开夏威夷昼夜采样计划（HaDS）的调研数据集：该数据集针对夏威夷瓦胡岛迎风海岸的卡内奥赫湾及其邻近远海两处生境，以每1.5小时一次的高分辨率采样频率，对表层海洋微生物群落开展了为期48小时的连续采样。2021年8月，研究团队在卡内奥赫湾沿岸站位（HP1）及邻近远海站位（STO1）的48小时周期内，共计完成33个时间点的采样，并同步产出了59份宏转录组（metatranscriptomes）、65份短读长宏基因组（short-read metagenomes）、8份长读长宏基因组（long-read metagenomes）以及66份转运RNA（transfer RNA）转录文库，同时配套获取了海洋生物地球化学相关检测数据。此外，研究团队借助卡内奥赫湾常规时间序列采样项目，获取了HaDS开展前的晚秋及春季采集的样本，并从中生成了4份深度测序的短读长宏基因组（Tucker等，2021；Tucker等，2025）。本项目的核心目标为解析海洋微生物对海洋生物地球化学要素昼夜变化的响应特征，并探究转录与翻译调控的潜在机制。本数据集由亥姆霍兹功能海洋生物多样性研究所的A. Murat Eren博士负责采集。更多相关信息可访问链接：https://merenlab.org/data/hads/，或参阅待发表的数据集描述论文（Tucker等，待刊）。时间戳采用协调世界时（UTC）。