Inhibition potential of Caryocar brasiliense on methicillin-resistant Staphylococcus pseudintermedius isolated from the ocular surface of dogs with ophthalmopathies

ABSTRACT The indiscriminate use of antibiotics has contributed to the emergence of multiresistant bacteria. Faced with this, the search for antibiotics from plants has proven to be a promising alternative. The objective of this work was to isolate and identify Staphylococcus sp. resistant to methicillin of the ocular surface of dogs with ophthalmopathies and to evaluate its susceptibility to alcoholic extract of the bark and hexane extract of the pulp of Caryocar brasiliense. Biological material was collected from the ocular surface of 21 dogs presenting ophthalmopathies. We isolated 64 S. pseudintermedius, among these, 4 isolates were identified as methicillin-resistant S. pseudintermedius (MRSP). The alcoholic extract of C. brasiliense peel was able to inhibit the bacterial growth of MRSP isolates at a concentration of 2.2%. Thus, the extract from the C. brasiliense peel has antimicrobial potential and represents an alternative in the control of MRSP.

摘要：抗生素的滥用催生了多重耐药细菌的出现。在此背景下，从植物中筛选新型抗生素已被证实为极具前景的替代方案。本研究旨在从罹患眼病的犬只眼表中分离并鉴定耐甲氧西林葡萄球菌属（Staphylococcus sp.）菌株，并评估其对巴西核果木（Caryocar brasiliense）树皮乙醇提取物及果肉正己烷提取物的敏感性。本研究采集了21例眼病犬的眼表生物样本，共分离获得64株中间葡萄球菌（S. pseudintermedius），其中4株被鉴定为耐甲氧西林中间葡萄球菌（MRSP）。浓度为2.2%的巴西核果木树皮乙醇提取物即可抑制耐甲氧西林中间葡萄球菌分离株的生长。由此可见，巴西核果木树皮提取物具备抗菌活性潜力，可作为耐甲氧西林中间葡萄球菌防控的潜在替代方案。