Reprogramming of chromatin accessibility in somatic cell nuclear transfer is DNA replication independent. Reprogramming of chromatin accessibility in somatic cell nuclear transfer is DNA replication independent

Mammalian oocytes have the ability to reset the transcriptional program of differentiated somatic cells into that of totipotent embryos through somatic cell nuclear transfer (SCNT). However, the mechanisms underlying SCNT-mediated reprogramming are largely unknown. To understand the mechanisms governing chromatin reprogramming during SCNT, we profiled DNaseI hypersensitive sites (DHSs) in donor cumulus cells and 1-cell stage SCNT embryos. To our surprise, the chromatin accessibility landscape of the donor cells is drastically changed to recapitulate that of the in vitro fertilization (IVF)-derived zygotes within 12 hours. Interestingly, this DHS reprogramming takes place even in the presence of a DNA replication inhibitor, suggesting that SCNT-mediated DHS reprogramming is independent of DNA replication. Thus, the study not only reveals the rapid and drastic nature of the changes in chromatin accessibility through SCNT, but also provides a DNA replication-independent model for studying cellular reprogramming. Overall design: Here we performed low-input DNase-Seq (liDNase-Seq) profilling of Donor cumulus cells, 1-Cell stage somantic nuclear Transfer (SCNT) embryos and in 1-Cell Aphidicolin treated SCNT embryos