Identification and validation of a tumor-infiltrating Treg transcriptional signature conserved across species and tumor types.

We reported transcriptional characterization of tumor-infiltrating T regulatory cells (TITRs) that is shared between species and among different tumor types and stages. We genomically profiled immunocytes from 2 species: 1. Mouse: CD4+ Treg and Tconv, and CD8+ T cells from tumor and spleen of B16, MC38 or CT26 tumor bearing mice, as well as from spleens of non-tumor bearing mice. 2. Human: CD4+ Treg cells from surgically resected and cryopreserved human colorectal carcinomas or normal colon tissue. Overall design: Gene expression profiles of CD4+ Treg and Tconv, and CD8+ T cells from tumor and spleen of B16, MC38 or CT26 tumor bearing mice (biological triplicates), as well as from spleens of non-tumor bearing mice, and CD4+ Treg cells from 12 surgically resected and cryopreserved human colorectal carcinomas or normal colon tissue.