HyReCo - Hybrid re-stained and consecutive histological serial sections

This dataset was acquired at the Radboud University Medical Center, Nijmegen, the Netherlands and enriched with landmarks by Fraunhofer MEVIS. It consists of nine datasets of consecutive sections, each containing four slides stained with H&E, CD8, CD45, Ki67, respectively. In addition, PHH3-stained slides have been produced by removing the cover slip from the H&E-stained slide, bleaching the H&E stain, re-staining the same section with PHH3 and scanning it again, similar to the T-CyCIF technique [@gerdes_highly_2013; @lin_highly_2018] that is well established in fluorescence imaging. For each of these sections , 11--19 landmarks (138 per stain, 690 in total) have been placed manually on corresponding structures and verified by two experienced researchers.

本数据集采集于荷兰奈梅亨拉德堡德大学医学中心（Radboud University Medical Center），并由弗劳恩霍夫医学影像研究所（Fraunhofer MEVIS）添加了地标标记。数据集包含9组连续切片数据集，每组均包含4张分别经苏木精-伊红（H&E）、CD8、CD45及Ki67染色的病理切片。此外，研究人员参照荧光成像领域已成熟应用的T-CyCIF技术[@gerdes_highly_2013; @lin_highly_2018]，通过移除H&E染色切片的盖玻片、褪去苏木精-伊红染色液、使用PHH3对同一切片进行复染并重新扫描，制备了PHH3染色切片。针对每一张上述切片，研究人员已在对应结构上手动标注了11至19个地标点（单种染色方式共138个地标点，总计690个地标点），并经两名经验丰富的研究人员核验。

搜集汇总

背景与挑战

背景概述

HyReCo数据集是一个用于计算病理学和图像配准研究的组织学图像数据集，包含连续切片和再染色切片。它由九个病例组成，每个病例有H&E、CD8、CD45、Ki67染色的连续切片，以及H&E切片再染色为PHH3的切片，并提供了手动标注的地标点以支持图像对齐。此外，数据集还包括一个额外的再染色图像对集合，用于提高注释准确性，适用于医学图像分析任务。