Time-course of the cell surface expression of human DAT variants in COS-7 cells.

COS-7 cells were transiently transfected with FL DAT or hDATΔEX6. After 1, 2, and 3 days, cells were incubated with sulfo-NHS-SS-biotin for 120 min at 4°C, and then processed for the isolation of biotinylated hDAT in the plasma membrane. Western blotting with the hDAT-specific antibody demonstrated the cell surface expression of 80 kDa and 50 kDa hDATΔEX6 in association with FL hDAT. The data represent a typical result from one experiment followed by 2 additional experiments with similar results.